RHEB Protein, Human (GST)

RHEB is an important small GTPase that acts as an allosteric activator of the mTORC1 complex, a key nutrient sensor that controls cell growth and biomass production. In response to signals such as nutrients and growth factors, RHEB activates the MTOR kinase within mTORC1 by inducing conformational changes. RHEB Protein, Human (GST) is the recombinant human-derived RHEB protein, expressed by E. coli , with N-GST labeled tag. The total length of RHEB Protein, Human (GST) is 184 a.a., with molecular weight of ~45.0 kDa.

RHEB, a small GTPase, functions as a crucial allosteric activator of the canonical mTORC1 complex, a pivotal nutrient sensor orchestrating cellular biomass generation and growth. In response to cues such as nutrients, growth factors, or amino acids, RHEB plays a specific role in activating the protein kinase activity of MTOR within the mTORC1 complex. This activation is achieved through inducing a conformational change that aligns residues in the active site of MTOR, thereby enhancing the phosphorylation of key downstream effectors like ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). Additionally, RHEB is essential for the localization of the TSC-TBC complex to lysosomal membranes, providing spatial regulation to mTORC1 activity. Conversely, during starvation, the TSC-TBC complex inactivates RHEB, preventing mTORC1 activation and contributing to cellular adaptation under nutrient-deprived conditions. Notably, RHEB exhibits low intrinsic GTPase activity, further emphasizing its role in dynamically regulating mTORC1 signaling.

Human

E. coli

N-GST

Q15382 (M1-M184)

6009  [NCBI]

MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Approximately 45.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 8% Trehalose, 4% Mannitol, 50 mM NaCl, 10 mM GSH, 0.05% Tween 80, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.