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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. RIOK2 Protein, Human (Sf9, His, GST)

RIOK2 Protein, Human (Sf9, His, GST)

Cat. No.: HY-P701766
COA Handling Instructions

RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (Sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of RIOK2 Protein, Human (Sf9, His, GST) is 551 a.a., .

For research use only. We do not sell to patients.

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10 μg $320 In-stock
50 μg $900 In-stock
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Description

RIOK2 is an important serine/threonine protein kinase that is critical in the final cytoplasmic maturation phase of the 40S ribosomal subunit. It promotes the export of 40S preribosomal particles from the nucleus to the cytoplasm, which is essential for ribosome biogenesis. RIOK2 Protein, Human (Sf9, His, GST) is the recombinant human-derived RIOK2 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of RIOK2 Protein, Human (Sf9, His, GST) is 551 a.a., .

Background

RIOK2, a serine/threonine-protein kinase, plays a pivotal role in the final stages of cytoplasmic maturation of the 40S ribosomal subunit. Its involvement extends to the export of pre-40S ribosome particles from the nucleus to the cytoplasm, a crucial step in ribosomal biogenesis. The kinase activity of RIOK2 is essential for orchestrating the release of NOB1, PNO1, and LTV1 from the late pre-40S subunit and facilitating the processing of 18S-E pre-rRNA into mature 18S rRNA. Beyond its role in ribosome maturation, RIOK2 exhibits regulatory functions in the cell cycle, specifically influencing the timing of the metaphase-anaphase transition during mitotic progression. This regulatory role is likely modulated by phosphorylation, possibly by PLK1, highlighting the multifaceted contributions of RIOK2 to cellular processes.

Species

Human

Source

Sf9 insect cells

Tag

N-8*His;N-GST

Accession

Q9BVS4 (G2-E552)

Gene ID

55781

Molecular Construction
N-term
8*His-GST
RIOK2 (G2-E552)
Accession # Q9BVS4
C-term
Synonyms
RIOK2; Serine/threonine-protein kinase RIO2; RIO kinase 2
AA Sequence

GKVNVAKLRYMSRDDFRVLTAVEMGMKNHEIVPGSLIASIASLKHGGCNKVLRELVKHKLIAWERTKTVQGYRLTNAGYDYLALKTLSSRQVVESVGNQMGVGKESDIYIVANEEGQQFALKLHRLGRTSFRNLKNKRDYHKHRHNVSWLYLSRLSAMKEFAYMKALYERKFPVPKPIDYNRHAVVMELINGYPLCQIHHVEDPASVYDEAMELIVKLANHGLIHGDFNEFNLILDESDHITMIDFPQMVSTSHPNAEWYFDRDVKCIKDFFMKRFSYESELFPTFKDIRREDTLDVEVSASGYTKEMQADDELLHPLGPDDKNIETKEGSEFSFSDGEVAEKAEVYGSENESERNCLEESEGCYCRSSGDPEQIKEDSLSEESADARSFEMTEFNQALEEIKGQVVENNSVTEFSEEKNRTENYNRQDGQRVQGGVPAGSDEYEDECPHLIALSSLNREFRPFRDEENVGAMNQYRTRTLSITSSGSAVSCSTIPPELVKQKVKRQLTKQQKSAVRRRLQKGEANIFTKQRRENMQNIKSSLEAASFWGE

Molecular Weight

Approximately 90.8 KDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50mM HEPES (pH 7.5), 200mM NaCl, 10% glycerol, 5mM DTT, 0.1M Trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

RIOK2 Protein, Human (Sf9, His, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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RIOK2 Protein, Human (Sf9, His, GST)
Cat. No.:
HY-P701766
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