RNF114 Protein, Human

The RNF114 protein is an E3 ubiquitin protein ligase that critically regulates a variety of cellular processes by ubiquitinating various substrates. RNF114 is involved in cell cycle progression, apoptosis, osteoclastogenesis and immunity, serving as a negative regulator of NF-kappa-B-dependent transcription, affecting immune responses by ubiquitinating TNFAIP3 and potentially TRAF6. RNF114 Protein, Human is the recombinant human-derived RNF114 protein, expressed by E. coli , with tag free. The total length of RNF114 Protein, Human is 227 a.a., .

RNF114 Protein, functioning as an E3 ubiquitin-protein ligase, plays a pivotal role in orchestrating diverse cellular processes by promoting the ubiquitination of various substrates. With broad implications, it is involved in the regulation of fundamental biological processes including cell cycle progression, apoptosis, osteoclastogenesis, as well as both innate and adaptive immunity. RNF114 acts as a negative regulator of NF-kappa-B-dependent transcription by facilitating the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3, and potentially TRAF6, thereby influencing immune responses. Additionally, it functions as a negative regulator of T-cell activation and impedes cellular dsRNA responses and interferon production by targeting the MAVS component for proteasomal degradation. Through its ubiquitination of CDK inhibitors, such as CDKN1A, CDKN1B, and CDKN1C, RNF114 stimulates G1-to-S phase transition, suppressing cellular senescence and potentially contributing to spermatogenesis. This multifaceted activity underscores the significance of RNF114 in governing key cellular pathways and processes.

Human

E. coli

Tag Free

Q9Y508 (A2-Q228)

55905

Approximately 25.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 5% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.