RNF135 Protein, Human (His)

The RNF135 protein acts as an E2-dependent E3 ubiquitin protein ligase and serves as a RIGI coreceptor for viral RNA sensing and activation of antiviral innate immune responses. RNF135 Protein, Human (His) is the recombinant human-derived RNF135 protein, expressed by E. coli , with N-6*His labeled tag. The total length of RNF135 Protein, Human (His) is 431 a.a., .

RNF135, as an E2-dependent E3 ubiquitin-protein ligase, plays a pivotal role in the innate immune response by serving as a coreceptor for RIGI in the detection of viral RNAs within the cell cytoplasm. Collaborating with the UBE2D3, UBE2N, and UB2V1 E2 ligases, RNF135 orchestrates the 'Lys-63'-linked polyubiquitination of RIGI, which is crucial for the activation of the RIG-I signaling pathway during viral infection. Additionally, through a ubiquitin-independent mechanism, RNF135 facilitates the bridging of RIGI filaments formed on longer viral RNAs, further enhancing RIG-I pathway activation. This dual mechanism, involving both ubiquitin-dependent and -independent processes, suggests a sophisticated regulation of the RIG-I signaling pathway, potentially influenced by the length of the viral RNA. Furthermore, in association with the E2 ligase UBE2N, RNF135 constitutively generates unanchored 'Lys-63'-linked polyubiquitin chains, providing an alternative means to activate the RIG-I signaling pathway and emphasizing the complexity of RNF135's involvement in antiviral immune responses.

Human

E. coli

N-6*His

Q8IUD6 (A2-V432)

84282

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.