RNF146 Protein, Human (His)

RNF146 protein is an E3 ubiquitin protein ligase that specifically recognizes and binds poly-ADP-ribosylated (PARsylated) proteins and coordinates their ubiquitination and degradation. Its critical roles span cell survival, DNA damage response, and activation of Wnt signaling. RNF146 Protein, Human (His) is the recombinant human-derived RNF146 protein, expressed by E. coli , with N-6*His labeled tag. The total length of RNF146 Protein, Human (His) is 358 a.a., .

RNF146 Protein, functioning as an E3 ubiquitin-protein ligase, exhibits a specialized role in recognizing and binding poly-ADP-ribosylated (PARsylated) proteins, orchestrating their ubiquitination, and subsequent degradation. This molecular mechanism positions RNF146 at the nexus of crucial biological processes, including cell survival and the DNA damage response. Particularly noteworthy is its function as an activator of the Wnt signaling pathway, achieved through mediating the ubiquitination of PARsylated AXIN1 and AXIN2, key components of the beta-catenin destruction complex. Operating in tandem with tankyrase proteins (TNKS and TNKS2), RNF146 targets various proteins, including AXIN1, AXIN2, BLZF1, CASC3, TNKS, and TNKS2, recognizing and binding them via its WWE domain and subsequently promoting their ubiquitination for degradation. The observed ubiquitin linkage types vary, with TNKS2 undergoing ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is exclusively ubiquitinated at 'Lys-48'. Beyond its role in Wnt signaling, RNF146 emerges as a neuroprotective protein, safeguarding against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia by disrupting PAR-induced cell death (parthanatos). Additionally, it plays a crucial role in cellular responses to DNA damage, promoting DNA repair, cell survival post-gamma irradiation, and rescuing cells from G1 arrest induced by DNA-damaging agents like N-methyl-N-nitro-N-nitrosoguanidine (MNNG). This multifaceted functionality underscores the significance of RNF146 in maintaining cellular integrity and response to diverse challenges.

Human

E. coli

N-6*His

Q9NTX7 (M2-V359)

81847

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.