1. Recombinant Proteins
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. RNF181 Protein, Human

RNF181 Protein, an E3 ubiquitin-protein ligase, mediates the monoubiquitination of the 26S proteasome subunit PSMC2/RPT1. This suggests a role in regulating the 26S proteasome, a vital cellular machinery for protein degradation. RNF181's capacity to modulate PSMC2/RPT1 implies involvement in the intricate regulatory network governing cellular protein homeostasis. RNF181 Protein, Human is the recombinant human-derived RNF181 protein, expressed by E. coli , with tag free. The total length of RNF181 Protein, Human is 152 a.a., .

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Description

RNF181 Protein, an E3 ubiquitin-protein ligase, mediates the monoubiquitination of the 26S proteasome subunit PSMC2/RPT1. This suggests a role in regulating the 26S proteasome, a vital cellular machinery for protein degradation. RNF181's capacity to modulate PSMC2/RPT1 implies involvement in the intricate regulatory network governing cellular protein homeostasis. RNF181 Protein, Human is the recombinant human-derived RNF181 protein, expressed by E. coli , with tag free. The total length of RNF181 Protein, Human is 152 a.a., .

Background

RNF181 Protein, functioning as an E3 ubiquitin-protein ligase, operates by accepting ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and subsequently directly transferring ubiquitin to its targeted substrates. Notably, RNF181 catalyzes the monoubiquitination of the 26S proteasome subunit PSMC2/RPT1. This activity suggests a role in the regulation of the 26S proteasome, a crucial cellular machinery responsible for protein degradation. The ability of RNF181 to mediate monoubiquitination of PSMC2/RPT1 implies potential involvement in the modulation of proteasomal function, contributing to the intricate regulatory network governing cellular protein homeostasis.

Species

Human

Source

E. coli

Tag

Tag Free

Accession

Q9P0P0 (A2-T153)

Gene ID

51255

Molecular Construction
N-term
RNF181 (A2-T153)
Accession # Q9P0P0
C-term
Synonyms
RNF181; E3 ubiquitin-protein ligase RNF181; RING finger protein 181
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
RNF181 Protein, Human
Cat. No.:
HY-P701560
Quantity:
MCE Japan Authorized Agent: