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  4. S6K1/RPS6KB1 Protein, Human (sf9, GST)

S6K1/RPS6KB1 Protein, Human (sf9, GST)

Cat. No.: HY-P76588
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S6K1/RPS6KB1 is a kinase downstream of mTOR that regulates cellular processes critical for proliferation, growth, and cell cycle. It phosphorylates EIF4B, RPS6 and EEF2K and regulates protein synthesis by inhibiting the pro-apoptotic function of BAD, thereby enhancing cell survival. S6K1/RPS6KB1 Protein, Human (sf9, GST) is the recombinant human-derived S6K1/RPS6KB1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.

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50 μg $280 In-stock
100 μg   Get quote  

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  • Biological Activity

  • Technical Parameters

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Description

S6K1/RPS6KB1 is a kinase downstream of mTOR that regulates cellular processes critical for proliferation, growth, and cell cycle. It phosphorylates EIF4B, RPS6 and EEF2K and regulates protein synthesis by inhibiting the pro-apoptotic function of BAD, thereby enhancing cell survival. S6K1/RPS6KB1 Protein, Human (sf9, GST) is the recombinant human-derived S6K1/RPS6KB1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

Background

S6K1/RPS6KB1, a serine/threonine-protein kinase, functions downstream of mTOR signaling in response to growth factors and nutrients, orchestrating cellular processes crucial for proliferation, cell growth, and cell cycle progression. Through its phosphorylation of EIF4B, RPS6, and EEF2K, it regulates protein synthesis, contributing to cell survival by suppressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex, and upon mitogenic stimulation, phosphorylation by mTORC1 leads to dissociation and activation. S6K1 also plays a pivotal role in translation initiation by phosphorylating substrates in the pre-initiation complex. Furthermore, it controls translation elongation by phosphorylating EEF2 kinase in response to IGF1. Additionally, S6K1 is involved in feedback regulation of mTORC2 and mTORC1, mediating cell survival, and modulating insulin resistance. Notably, it participates in the regulation of fatty acid uptake and interferon-gamma-induced translation inhibition through its phosphorylation of EPRS. These multifaceted roles highlight the significance of S6K1 in coordinating diverse cellular responses to environmental cues.

Biological Activity

No Kinase Activity.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

P23443-1 (M1-L525)

Gene ID
Molecular Construction
N-term
GST
S6K1 (M1-L525)
Accession # P23443-1
C-term
Synonyms
Ribosomal protein S6 kinase beta-1; S6K1; P70S6K1; p70 S6KA; RPS6KB1; STK14A
Molecular Weight

Approximately 96 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol, 1 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

S6K1/RPS6KB1 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
S6K1/RPS6KB1 Protein, Human (sf9, GST)
Cat. No.:
HY-P76588
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