1. Recombinant Proteins
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 3C-like Protease
  5. SARS-CoV-2 3C-like Proteinase (N-His)

SARS-CoV-2 3C-like Proteinase (N-His)

Cat. No.: HY-P7429A
Handling Instructions

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus.

For research use only. We do not sell to patients.

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Free Sample   Apply now
5 μg $62 In-stock
10 μg $100 In-stock
50 μg $280 In-stock
100 μg $450 In-stock
500 μg $1250 In-stock
1 mg $2000 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SARS-CoV-2 3C-like Proteinase (His), His, expressed in E.coli, mediates viral polyprotein maturation and multiple cleavages of host proteins to modulate viral translation and transcription with a His tag at the N-terminus[1].

Background

The 3C protease has been regarded as a valid target due to its structural similarity among different picornaviruses and minimal sequence similarity with host proteins; therefore, the development of potent inhibitors against the 3C protease as an antiviral drug is ongoing. Duck hepatitis A virus (DHAV) belongs to the Picornavidea family and is a major threat to the poultry industry[1].

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Dabcyl- KTSAVLQSGFRKME-Edans (Ssynthetic). The specific activity is >13000 pmol/min/mg.

Species

Virus

Source

E. coli

Tag

N-His

Accession

YP_009725301.1 (S1-Q306)

Gene ID

43740578

Molecular Construction
N-term
6*His
SARS-CoV-2 3CLpro (S1-Q306)
Accession # YP_009725301.1
C-term
Synonyms
M Proteinase; 3CL Proteinase; 2019-nCoV 3C-like Proteinase
AA Sequence

SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ

Molecular Weight

Approximately 34 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose, 0.02% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

SARS-CoV-2 3C-like Proteinase (N-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SARS-CoV-2 3C-like Proteinase (N-His)
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HY-P7429A
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