Semaphorin-3A/SEMA3A Protein, Human (HEK293, His-Flag)

Semaphorin-3A/SEMA3A proteins are critical for shaping the olfactory system and influence puberty-related neuronal control. SEMA3A is known for inducing neuronal growth cone collapse, which leads to the formation of complex neuronal circuits by guiding the growth cone through a motor inhibitory mechanism. Semaphorin-3A/SEMA3A Protein, Human (HEK293, His-Flag) is the recombinant human-derived Semaphorin-3A/SEMA3A protein, expressed by HEK293 , with N-6*His, N-Flag labeled tag. The total length of Semaphorin-3A/SEMA3A Protein, Human (HEK293, His-Flag) is 521 a.a., with molecular weight of 70-75 kDa.

The Semaphorin-3A/SEMA3A Protein plays a crucial role in the development of the olfactory system and exerts influence over neuronal control of puberty. Known for its capacity to induce the collapse and paralysis of neuronal growth cones, SEMA3A is implicated in guiding specific growth cones through a motility-inhibiting mechanism, thereby contributing to the intricacies of neuronal circuit formation. This protein serves as a ligand by binding to the complex neuropilin-1/plexin-1, underscoring its involvement in transducing signaling cues critical for neuronal guidance. Additionally, SEMA3A interacts with PLXND1, further highlighting its multifaceted role in mediating cellular interactions during the intricate processes of olfactory system development and the regulation of puberty-related neuronal functions.

Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 is coated at 2 µg/mL can bind Human Semaphorin 3A. The ED₅₀ for this effect is 198.4 ng/mL.

Human

HEK293

N-6*His;N-Flag

Q14563 (K26-F546)

10371  [NCBI]

KNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLYVGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLVNKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRRVFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRPIVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAISAMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYF

Approximately 70-75 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4 or 20mM Tris-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH 8.5 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.