Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His)

Semaphorin-3C (SEMA3C) protein plays a crucial role in developmental processes by binding to plexin family members, particularly PLXND1, orchestrating intricate cellular signaling pathways. Its indispensable contribution to normal cardiovascular development during embryogenesis and its function as an attractant for growing axons underscore SEMA3C's significance in axon growth and guidance. Semaphorin-3C/SEMA3C Protein, Human (HEK293, C-His) is the recombinant human-derived Semaphorin-3C/SEMA3C, expressed by HEK293 , with C-6*His labeled tag. ,

Semaphorin-3C (SEMA3C) protein serves as a pivotal regulator in developmental processes by binding to members of the plexin family. Crucially, SEMA3C is indispensable for normal cardiovascular development in embryogenesis. Its functionality extends to acting as an attractant for growing axons, thereby playing a significant role in axon growth and guidance. In molecular interactions, SEMA3C forms a specific association with PLXND1, highlighting its involvement in intricate cellular signaling pathways that contribute to the orchestration of developmental events.

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells to Fibronectin. Recombinant Human Semaphorin 3C immobilized at 10 μg/mL, with 2 ng/mL rhFN-1, 100 μL/well, will induce approximately 70.40% adhesion.

Human

HEK293

C-6*His

Q99985-1 (G21-S738)

10512

GSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGSKDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCTYLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRTDQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKWTTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFNGPFAHKEGPNHQLISYQGRIPYPRPGTCPGGAFTPNMRTTKEFPDDVVTFIRNHPLMYNSIYPIHKRPLIVRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNNSVSGELILEELEVFKNHAPITTMKISSKKQQLYVSSNEGVSQVSLHRCHIYGTACADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNPLTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLIRSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHSEMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINS

Approximately 65-72 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.