Semaphorin-7A/SEMA7A Protein, Human (HEK293, His)

Semaphorin-7A (SEMA7A) plays a key role in integrin-mediated signaling, affecting multiple cellular functions, including cell migration and immune responses. SEMA7A promotes focal adhesion complex formation, activates PTK2/FAK1 and induces phosphorylation of MAPK1 and MAPK3. Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-7A/SEMA7A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-7A/SEMA7A Protein, Human (HEK293, His) is 604 a.a., with molecular weight of 70-75 kDa.

The Semaphorin-7A (SEMA7A) protein assumes a crucial role in integrin-mediated signaling, exerting its influence on diverse cellular functions that include the regulation of cell migration and immune responses. SEMA7A actively promotes the formation of focal adhesion complexes, activating protein kinase PTK2/FAK1 and subsequently inducing the phosphorylation of MAPK1 and MAPK3. Furthermore, it plays a significant role in orchestrating the production of pro-inflammatory cytokines by monocytes and macrophages, thereby modulating inflammation and T-cell-mediated immune responses. In developmental contexts, SEMA7A is implicated in promoting axon growth in the embryonic olfactory bulb and facilitates attachment, spreading, and dendrite outgrowth in melanocytes. Its intricate molecular interactions involve associations with integrins ITGA1 and ITGB1, suggesting its involvement in mediating cell adhesion, and it interacts with PLXNC1, adding to the complexity of its regulatory role in diverse cellular processes.

Human

HEK293

C-His

O75326 (Q45-A648)

8482  [NCBI]

70-75 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.