Serpin D1 Protein, Human (HEK293, His)

Serpin D1 protein is a thrombin inhibitor activated by glycosaminoglycans such as heparin or dermatan sulfate. It replaces antithrombin III and becomes the main thrombin inhibitor in the presence of dermatan sulfate. In addition to thrombin inhibition, serpin D1 also has glycosaminoglycan-independent chymotrypsin inhibitory activity. Serpin D1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin D1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin D1 Protein, Human (HEK293, His) is 480 a.a., with molecular weight of ~75-80 kDa.

Serpin D1 Protein functions as a thrombin inhibitor, with its activity activated by glycosaminoglycans such as heparin or dermatan sulfate. In the presence of dermatan sulfate, HC-II becomes the predominant thrombin inhibitor, displacing antithrombin III (AT-III). Beyond its role in thrombin inhibition, Serpin D1 also exhibits chymotrypsin-inhibiting activity, independent of glycosaminoglycans. Notably, peptides at the N-terminal of HC-II demonstrate chemotactic activity for both monocytes and neutrophils. This multifaceted functionality highlights the diverse roles of Serpin D1 in regulating protease activities and participating in immune responses, underscoring its significance in maintaining hemostasis and modulating inflammatory processes.

Measured by its ability to inhibit Thrombin cleavage of a peptide substrate, Boc-VPR-AMC. The IC₅₀ value is 0.344 nM, as measured under the described conditions.

Human

HEK293

C-His

P05546 (G20-S499)

3053  [NCBI]

GSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFLFLIYEHRTSCLLFMGRVANPSRS

Approximately 75-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.