Serpin G1 Protein, Rat (HEK293, His)

Serpin G1 protein binds complement and inhibits serine-type endopeptidases. It is located in the extracellular space and is involved in aging. Serpin G1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin G1 protein, expressed by HEK293 , with C-His labeled tag.

The Serpin G1 protein is predicted to possess complement binding activity and serine-type endopeptidase inhibitor activity. It plays a role in aging and is located in the extracellular space. This protein has been utilized in the study of glioblastoma and serves as a biomarker for acute myocardial infarction and acute necrotizing pancreatitis. In humans, the ortholog(s) of this gene have been implicated in various diseases, including COVID-19, angioedema, cerebral infarction, pancreatitis, and toxic shock syndrome. The human ortholog of this gene is SERPING1, a member of the serpin family G. The Serpin G1 protein exhibits biased expression in the liver, lung, and seven other tissues.

Measured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC₅₀ value is 6.646 nM, as measured under the described conditions.

Rat

HEK293

C-His

Q6P734/NP_954524.1 (D23-A504)

295703  [NCBI]

DSEVTSHSSQDPLVVQEGSRDSVPERDGSRSPIEHTGQSSTWPTTSGSTKISNDTMDQVANESFIQHVQPAAQLPEDSPSQSPVNSSSPPSTASAPPTQAPTEPLCPEPLAWCSDSDRDSSEATLSEALTDFSVKLYHAFSATKKAETNMAFSPFSIASLLTQVLLGAGDSTKSNLEDILSYPKDFACVHQTLKAFSSKGVTSVSQIFHSPDLAIRDTYVNASLSLYGSSPRVLGPDGDANLKLINTWVAENTNHKINELLDSLPSDTRLVLLNAVYLSAKWKKTFEQKKMMASFLYKNSMIKVPMLSSKKYPLALFNDQTLKAKVGQLQLSHNLSFVIMVPQSPTHQLEDMEKALNPTVFKAILKKLELSKFQPTYVMMPRIKVKSSQDMLSIMEKLEFFDFTYDLNLCGLTEDPDLQVSSMKHETVLELTETGVEAAAASTISVARNLLIFEVQQPFLFLLWDQRHKFPVFMGRVYDPRA

Approximately 70-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE. The protein migrates as a 70-95 kDa band under reducing SDS-PAGE due to glycosylation.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.