Serum Albumin/ALB Protein, Human (Biotinylated, HEK293, His-Avi)

Serum Albumin/ALB Protein has versatile binding capabilities, binding water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs. Its primary role involves regulating blood colloidal osmotic pressure for homeostasis. ALB acts as a major transporter for zinc, binding 80% of plasma zinc, and 45% of circulating calcium and magnesium. It interacts with the bacterial siderophore enterobactin, inhibiting E. coli's iron uptake. ALB also engages with FCGRT, regulating homeostasis, and forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin, highlighting diverse molecular interactions and maintaining functionality in fatty acid binding. Serum Albumin/ALB Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Serum Albumin/ALB protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Serum Albumin/ALB Protein, Human (Biotinylated, HEK293, His-Avi) is 585 a.a., with molecular weight of 69-70 kDa.

The Serum Albumin/ALB protein serves a multifaceted role by binding to water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin, and drugs, indicative of its versatile binding capabilities. Its primary function likely revolves around the regulation of the colloidal osmotic pressure of blood, emphasizing its pivotal role in maintaining blood homeostasis. Notably, ALB acts as a major transporter for zinc, binding approximately 80% of all plasma zinc, and also serves as a significant transporter for calcium and magnesium, binding about 45% of circulating calcium and magnesium in plasma. The presence of potential calcium-binding sites suggests its involvement in calcium transport, with a distinct affinity rank order of zinc > calcium > magnesium. Additionally, ALB exhibits an special interaction with the bacterial siderophore enterobactin, inhibiting enterobactin-mediated iron uptake of E. coli and potentially limiting the utilization of iron by enteric bacteria. Furthermore, ALB engages in interactions with FCGRT, regulating its homeostasis, and forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin in plasma, highlighting its diverse roles in molecular interactions and maintaining its functionality in fatty acid binding.

Biotinylated Human Serum Albumin, His-Avi Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).

Human

HEK293

C-Avi;C-His

P02768 (D25-L609)

213  [NCBI]

DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

69-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.