Serum Albumin/ALB Protein, Mouse (HEK293, His)

Serum Albumin/ALB Protein binds to various substances such as water, calcium, sodium, potassium, fatty acids, hormones, bilirubin, and drugs. Its main function is to regulate blood osmotic pressure and transport zinc, calcium, and magnesium. ALB may interact with both zinc and calcium, potentially influencing their transport. It inhibits the uptake of iron by E.coli through enterobactin but not aerobactin. ALB also interacts with FCGRT and TASOR for homeostasis. It forms a complex with alpha-1-microglobulin without affecting fatty acid binding. Serum Albumin/ALB Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serum Albumin/ALB protein, expressed by HEK293, with C-His labeled tag. The total length of Serum Albumin/ALB Protein, Mouse (HEK293, His) is 584 a.a., with molecular weight of ~70.19 kDa.

Serum Albumin/ALB Protein is a versatile protein that binds to water, calcium, sodium, potassium, fatty acids, hormones, bilirubin, and drugs. Its primary role is to regulate the colloidal osmotic pressure of blood. It is the major transporter of zinc in plasma, binding to approximately 80% of all plasma zinc. Similarly, it is the major transporter of calcium and magnesium, binding to approximately 45% of circulating calcium and magnesium in plasma. It may have more than two calcium-binding sites and could also bind calcium in a non-specific manner. The shared binding site between zinc and calcium suggests a potential interaction and transport mechanism between these two elements in the blood. Additionally, ALB binds to the bacterial siderophore enterobactin, inhibiting its ability to facilitate iron uptake by E.coli from ferric transferrin. This limitation of iron utilization may restrict the growth of enteric bacteria such as E.coli. However, ALB does not hinder iron uptake facilitated by the bacterial siderophore aerobactin. ALB also interacts with FCGRT, regulating its own homeostasis, and interacts with TASOR. In plasma, it forms a covalently-linked complex with chromophore-bound alpha-1-microglobulin, but this interaction does not interfere with the binding of fatty acids to ALB.

Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-His

P07724 (E25-A608)

11657  [NCBI]

EAHKSEIAHRYNDLGEQHFKGLVLIAFSQYLQKCSYDEHAKLVQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNLRENYGELADCCTKQEPERNECFLQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYLHEVARRHPYFYAPELLYYAEQYNEILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRMKCSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECCHGDLLECADDRAELAKYMCENQATISSKLQTCCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVEDQEVCKNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTVLAEFQPLVEEPKNLVKTNCDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVGTKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTVDETYVPKEFKAETFTFHSDICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFAQFLDTCCKAADKDTCFSTEGPNLVTRCKDALA

Approximately 70.19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.