sFRP-3 Protein, Mouse (HEK293, His)

The sFRP-3 protein is a soluble Frizzled-related protein that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. Known as FRZB, it acts as a Wnt8 signaling antagonist with crucial effects on limb skeletogenesis. sFRP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived sFRP-3 protein, expressed by HEK293 , with C-6*His labeled tag.

sFRP-3, a soluble frizzled-related protein, operates as a modulator of Wnt signaling through its direct interaction with Wnts, contributing to the regulation of cell growth and differentiation in specific cell types. Notably, SFRP-3, also known as FRZB, plays a key role in limb skeletogenesis, functioning as an antagonist of Wnt8 signaling. Its regulatory influence extends to chondrocyte maturation and long bone development. In addition to its participation in Wnt signaling modulation, SFRP-3 interacts with MYOC, showcasing its engagement in molecular interactions associated with cellular processes. These diverse functions underscore the multifaceted regulatory role of sFRP-3 in Wnt-mediated pathways and skeletogenesis.

Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.797 µg/mL.

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  Measured by its ability to inhibit proliferation of MC3T3-E1. The ED₅₀ for this effect is 5.797μg/mL, corresponding to a specific activity is 172.503 units/mg.

Mouse

HEK293

C-6*His

P97401 (A33-S323)

20378

AACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAMEQFEGLLGTHCSPDLLFFLCAMYAPICTIDFQHEPIKPCKSVCERARQGCEPILIKYRHSWPESLACDELPVYDRGVCISPEAIVTADGADFPMDSSTGHCRGASSERCKCKPVRATQKTYFRNNYNYVIRAKVKEVKMKCHDVTAVVEVKEILKASLVNIPRDTVNLYTTSGCLCPPLTVNEEYVIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDMKLRHLGLGKTDASDSTQNQKSGRNSNPRPARS

Approximately 35-37 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.