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  4. NK Cell CD Proteins Macrophage CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins Siglec
  5. Siglec-3/CD33 Siglec-3/CD33
  6. Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi)

Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P701037
Handling Instructions

Siglec-3/CD33 protein, a sialic-acid-binding lectin, mediates cell-cell interactions and maintains immune cell quiescence. It prefers alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon ligand engagement, CD33's ITIMs phosphorylate, recruiting PTPN6/SHP-1 and PTPN11/SHP-2. These phosphatases, activated by Src-like kinases, regulate downstream pathways, including the repressive effect on monocyte activation through PI3K. CD33 also forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2, and engages C1QA via its C-terminus, activating inhibitory motifs. Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi) is 242 a.a., with molecular weight of 48-58 kDa.

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Description

Siglec-3/CD33 protein, a sialic-acid-binding lectin, mediates cell-cell interactions and maintains immune cell quiescence. It prefers alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon ligand engagement, CD33's ITIMs phosphorylate, recruiting PTPN6/SHP-1 and PTPN11/SHP-2. These phosphatases, activated by Src-like kinases, regulate downstream pathways, including the repressive effect on monocyte activation through PI3K. CD33 also forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2, and engages C1QA via its C-terminus, activating inhibitory motifs. Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi) is 242 a.a., with molecular weight of 48-58 kDa.
Background

The Siglec-3/CD33 protein, a sialic-acid-binding immunoglobulin-like lectin, plays a crucial role in mediating cell-cell interactions and maintaining immune cells in a resting state. It exhibits a preference for recognizing and binding alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement with ligands like C1q or sialylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within CD33's cytoplasmic tail undergo phosphorylation by Src-like kinases such as LCK. These phosphorylated ITIMs serve as docking sites for recruiting and activating protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, which, in turn, regulate downstream pathways through dephosphorylation of signaling molecules. CD33's repressive effect on monocyte activation involves phosphoinositide 3-kinase/PI3K. Structurally, the protein forms homodimers through disulfide linkages and interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. It also engages with C1QA via its C-terminus, activating CD33 inhibitory motifs.
Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

P20138-1 (D18-H259)
Gene ID

945  [NCBI]
Molecular Construction
N-term
CD33 (D18-H259)
Accession # P20138-1
His-Avi
C-term
Synonyms
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
Molecular Weight

48-58 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Siglec-3/CD33 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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