2. Recombinant Proteins
  3. CD Antigens Receptor Proteins Biotinylated Proteins
  4. Dendritic Cell CD Proteins Siglec
  5. Siglec-5/CD170 Siglec-5/CD170
  6. Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi)

Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P701039
Handling Instructions

Siglec-5 Protein, a putative adhesion molecule, participates in sialic-acid dependent cellular binding, showing equal affinity for alpha-2,3-linked and alpha-2,6-linked sialic acid. Its sialic acid recognition site may be masked through cis interactions with sialic acids on the same cell surface. Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-5 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi) is 418 a.a., with molecular weight of 70-80 kDa.

For research use only. We do not sell to patients.

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Description

Siglec-5 Protein, a putative adhesion molecule, participates in sialic-acid dependent cellular binding, showing equal affinity for alpha-2,3-linked and alpha-2,6-linked sialic acid. Its sialic acid recognition site may be masked through cis interactions with sialic acids on the same cell surface. Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Siglec-5 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi) is 418 a.a., with molecular weight of 70-80 kDa.
Background

Siglec-5 protein, a putative adhesion molecule, is implicated in sialic-acid dependent cellular binding, displaying equal affinity for both alpha-2,3-linked and alpha-2,6-linked sialic acid. Notably, the sialic acid recognition site of Siglec-5 may be subject to masking through cis interactions with sialic acids on the same cell surface.
Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

O15389 (E17-T434)
Gene ID
Molecular Construction
N-term
Siglec-5 (E17-T434)
Accession # O15389
His-Avi
C-term
Synonyms
CD170; CD33 antigen-like 2; CD33L2; OBBP2; Siglec5; SIGLEC5; OB-BP2
Molecular Weight

70-80 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Siglec-5 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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