Siglec-E Protein, Mouse (HEK293, Fc)

Siglec-E Protein is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke. Siglec-E Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-E protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-E Protein, Mouse (HEK293, Fc) is 336 a.a..

Sialic acid binding Ig-like lectin E (Siglec-E) is a mouse orthologue of human Siglec-9 and functions as a key immunosuppressive checkpoint molecule. Siglec-E is a sialic acid binding lectin predominantly expressed on the surface of myeloid cells to transduce inhibitory signal via recruitment of SH2-domain containing protein tyrosine phosphatase SHP-1/2 upon binding to its sialoglycan ligands. Siglec-E interacts with CD36 to inhibits downstream VAV signaling involved in modified LDL uptake, thereby delaying atherosclerosis. The endogenous inducible Siglec-E plays crucial anti-inflammatory and neuroprotective roles following ischemic stroke, and thus might underlie an intrinsic mechanism of resolution of inflammation and self-repair in the brain. Siglec-E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling and suppresses CD11b β2-integrin-dependent signaling^[1][2][3].

Measured by its binding ability in a functional ELISA. Immobilized Mouse Siglec-E at 2 μg/mL (100 μL/well) can bind biotinylated Mouse Galectin-3. The ED₅₀ for this effect is 0.2911 μg/mL.

Mouse

HEK293

C-hFc

Q6PJ50/AAH23280 (Q20-F355)

83382

QNPQEGFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGEPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTDVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMMMGTF

Approximately 78-100 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5 or PBS, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.