SIRP beta Protein, Mouse (HEK293, C-His)

SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins, Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs, resulting in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex. Sirpb is also implicated as a positive regulator of hematopoietic cells. SIRP beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived SIRP beta protein, expressed by HEK293 , with C-His labeled tag.

SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily with three IgSF domains in its extracellular region. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs. Ligation of Sirpb results in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex in RBL-2H3 cell transfectants. It also stimulated serotonin release from these cells. Sirpb is therefore implicated as a positive regulator of hematopoietic cells^[1][2].

Measured by its binding ability in a functional ELISA. Immobilized Mouse SIRP beta at 1μg/mL (100 μL/well) can bind Human SP-D (HY-P70986). The ED₅₀ for this effect is 722.6 ng/mL.

Mouse

HEK293

C-10*His

BAD26610 (A27-K363)

/

AMRELKVIQPVKSFFVGAGGSATLNCTVTSLLPVGPMRWYRGIGQSRLLIYSFTGEGFPRITNTSDTTKRNNMDFSIRISNVTPADSGTYYCVKFQRGPSDFYTEIQSGGGTELSVLAKPSSPMVSGPAARAVPQQTVTFTCRSHGFFPRNLTLKWFKNGDEISHLETSVEPEETSVSYRVSSTVQVVLEPRDVRSQIICEVDHVTLDRAPLRGIAHISEFIQVPPTLEIRQQPTMVWNVINVTCQIQKFYPPSFQLTWLENGNISRREVPFTLTVNKDGTYNWISCLLVNISALEENMVVTCQVEHDGQAEVIETHTVVVTEHQRVKELKTAGIAK

Approximately 54-77 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice