SMPD2 Protein, Human (Cell-Free, His)

In vitro experiments have demonstrated that the sphingomyelin phosphodiesterase 2 (SMPD2) protein plays a key role in catalyzing the hydrolysis of sphingomyelin, leading to the formation of ceramide and phosphocholine. Furthermore, SMPD2 exhibits its enzymatic activity by hydrolyzing 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (hemolytic platelet-activating factor) in vivo. SMPD2 Protein, Human (Cell-Free, His) is the recombinant human-derived SMPD2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SMPD2 Protein, Human (Cell-Free, His) is 423 a.a., with molecular weight of 49.1 kDa.

Sphingomyelin Phosphodiesterase 2 (SMPD2) is an enzyme that catalyzes the hydrolysis of sphingomyelin, cleaving it into ceramide and phosphocholine. This enzymatic activity is essential for sphingolipid metabolism and the regulation of cellular signaling. SMPD2 is also involved in the hydrolysis of 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine, specifically lyso-platelet-activating factor, in vivo. Additionally, SMPD2 acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine. The diverse substrate specificity of SMPD2 highlights its role in modulating the levels of bioactive lipid molecules, including ceramides and lysophospholipids, which are implicated in various cellular processes, including cell proliferation, apoptosis, and inflammation. Understanding the functions of SMPD2 provides insights into the intricate regulation of lipid metabolism and its impact on cellular homeostasis.

Human

E. coli Cell-free

N-10*His

O60906 (M1-Q423)

6610

MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHRGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGAGEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRTKEQ

49.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.