SMYD3 Protein, Human (sf9, GST)

SMYD3 Protein, a histone methyltransferase, specifically induces di- and tri-methylation of H3K4, avoiding monomethylation, and methylates H4K5. Crucial in transcriptional activation within an RNA polymerase complex, SMYD3 also exhibits DNA-binding affinity for sequences containing 5'-CCCTCC-3' or 5'-GAGGGG-3'. SMYD3 Protein, Human (sf9, GST) is the recombinant human-derived SMYD3 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of SMYD3 Protein, Human (sf9, GST) is 335 a.a., with molecular weight of ~58 kDa.

SMYD3 Protein, a histone methyltransferase, exhibits specificity in methylating 'Lys-4' of histone H3, inducing both di- and tri-methylation but not monomethylation (PubMed:15235609, PubMed:22419068). Additionally, it methylates 'Lys-5' of histone H4 (PubMed:22419068). Within an RNA polymerase complex, SMYD3 plays a crucial role in transcriptional activation (PubMed:15235609). The protein is noted for its ability to bind DNA sequences containing 5'-CCCTCC-3' or 5'-GAGGGG-3' (PubMed:15235609).

Human

Sf9 insect cells

N-GST

Q9H7B4-3 (K35-S369)

64754  [NCBI]

Approximately 58 kDa

Greater than 88% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 0.5 mM DTT, 0.5 mM GSH, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.