SOD1 Protein, Human (N-His)

SOD1 Protein, an enzyme crucial for cellular defense, operates as a superoxide dismutase, neutralizing radicals to safeguard cells from oxidative stress. Its enzymatic activity underscores the importance of SOD1 in maintaining cellular homeostasis and protecting biological systems from the harmful effects of reactive oxygen species accumulation. SOD1 Protein, Human (N-His) is the recombinant human-derived SOD1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of SOD1 Protein, Human (N-His) is 154 a.a., with molecular weight of ~20.0 kDa.

The subject, SOD1 Protein, functions as an enzyme crucial for cellular defense, as it plays a vital role in neutralizing radicals that are typically generated within cells and can be toxic to biological systems. Operating as a superoxide dismutase, SOD1 effectively destroys these radicals, thereby safeguarding cells from oxidative stress and potential damage caused by the accumulation of reactive oxygen species. The enzymatic activity of SOD1 underscores its importance in maintaining cellular homeostasis and protecting biological systems from the harmful effects of oxidative stress.

Specific actifity is 148.432 units/mg. When the inhibition percentage in the xanthine oxidase coupled reaction system mentioned above is 50%, the SOD enzyme activity in the reaction system is defined as one enzyme activity unit.

Human

E. coli

N-6*His

P00441 (A2-Q154)

6647

ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.