SPR Protein, Human (His)

SPR proteins play a key role in tetrahydrobiopterin biosynthesis by catalyzing the final one or two reductions required to form 5,6,7,8-tetrahydrobiopterin. This enzyme activity is critical for the production of tetrahydrobiopterin, a cofactor involved in various biochemical pathways, including neurotransmitter synthesis and nitric oxide production. SPR Protein, Human (His) is the recombinant human-derived SPR protein, expressed by E. coli , with N-6*His labeled tag. The total length of SPR Protein, Human (His) is 261 a.a., .

Sapropterin reductase (SPR) is an enzyme that plays a crucial role in the biosynthesis of tetrahydrobiopterin (BH4). Specifically, SPR catalyzes the final one or two reductions in the tetrahydrobiopterin biosynthetic pathway, ultimately forming 5,6,7,8-tetrahydrobiopterin. This co-factor is essential for the activity of aromatic amino acid hydroxylases, which are involved in the synthesis of neurotransmitters and other bioactive molecules. The catalytic activity of SPR is vital for maintaining appropriate levels of tetrahydrobiopterin, which, in turn, influences the proper functioning of various physiological processes, including neurotransmitter production. Dysregulation of BH4 biosynthesis can impact neurotransmitter homeostasis and has been implicated in certain neurological disorders. It has to succinctly outline SPR's role in the final steps of tetrahydrobiopterin biosynthesis, underscoring its importance in supporting crucial biochemical pathways.

Human

E. coli

N-6*His

P35270 (M1-K261)

6697

Approximately 30 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.