STC2/Stanniocalcin-2 Protein, Human (HEK293, His)

STC2/Stanniocalcin-2 Protein plays a vital role in maintaining calcium and phosphate homeostasis and exhibits strong anti-hypocalcemic effects. As a homodimer with disulfide linkages, STC2 plays a crucial structural role in regulating calcium and phosphate balance. This unique property emphasizes the pivotal role of STC2 in preserving the delicate equilibrium of essential minerals in the body. STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is the recombinant human-derived STC2/Stanniocalcin-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is 278 a.a., with molecular weight of 33-43 kDa.

STC2, a crucial player in calcium and phosphate homeostasis, exerts a potent anti-hypocalcemic action. Operating as a homodimer with disulfide linkages, STC2 showcases its structural significance in mediating regulatory responses related to the balance of calcium and phosphate levels. This distinctive property underscores the pivotal role of STC2 in maintaining the intricate equilibrium of essential minerals within the physiological milieu.

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a, corresponding to a specific activity is 4.308×10⁴ U/mg.

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  Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a, corresponding to a specific activity is 4.308×10⁴ U/mg

Human

HEK293

C-His

O76061 (T25-R302)

8614  [NCBI]

TDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWEDEQSEYSDIRR

33-43 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.