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STC2/Stanniocalcin-2 Protein, Human (HEK293, His)

Cat. No.: HY-P76096
COA Handling Instructions

STC2/Stanniocalcin-2 Protein plays a vital role in maintaining calcium and phosphate homeostasis and exhibits strong anti-hypocalcemic effects. As a homodimer with disulfide linkages, STC2 plays a crucial structural role in regulating calcium and phosphate balance. This unique property emphasizes the pivotal role of STC2 in preserving the delicate equilibrium of essential minerals in the body. STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is the recombinant human-derived STC2/Stanniocalcin-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is 278 a.a., with molecular weight of 33-43 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

STC2/Stanniocalcin-2 Protein plays a vital role in maintaining calcium and phosphate homeostasis and exhibits strong anti-hypocalcemic effects. As a homodimer with disulfide linkages, STC2 plays a crucial structural role in regulating calcium and phosphate balance. This unique property emphasizes the pivotal role of STC2 in preserving the delicate equilibrium of essential minerals in the body. STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is the recombinant human-derived STC2/Stanniocalcin-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of STC2/Stanniocalcin-2 Protein, Human (HEK293, His) is 278 a.a., with molecular weight of 33-43 kDa.

Background

STC2, a crucial player in calcium and phosphate homeostasis, exerts a potent anti-hypocalcemic action. Operating as a homodimer with disulfide linkages, STC2 showcases its structural significance in mediating regulatory responses related to the balance of calcium and phosphate levels. This distinctive property underscores the pivotal role of STC2 in maintaining the intricate equilibrium of essential minerals within the physiological milieu.

Biological Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a, corresponding to a specific activity is 4.308×104 U/mg.

  • Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 23.21 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a, corresponding to a specific activity is 4.308×104 U/mg
Species

Human

Source

HEK293

Tag

C-His

Accession

O76061 (T25-R302)

Gene ID
Molecular Construction
N-term
STC2 (T25-R302)
Accession # O76061
His
C-term
Synonyms
Stanniocalcin-2; STC-2; STC-related protein; STCRP
AA Sequence

TDATNPPEGPQDRSSQQKGRLSLQNTAEIQHCLVNAGDVGCGVFECFENNSCEIRGLHGICMTFLHNAGKFDAQGKSFIKDALKCKAHALRHRFGCISRKCPAIREMVSQLQRECYLKHDLCAAAQENTRVIVEMIHFKDLLLHEPYVDLVNLLLTCGEEVKEAITHSVQVQCEQNWGSLCSILSFCTSAIQKPPTAPPERQPQVDRTKLSRAHHGEAGHHLPEPSSRETGRGAKGERGSKSHPNAHARGRVGGLGAQGPSGSSEWEDEQSEYSDIRR

Molecular Weight

33-43 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

STC2/Stanniocalcin-2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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STC2/Stanniocalcin-2 Protein, Human (HEK293, His)
Cat. No.:
HY-P76096
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