SULT1B1 Protein, Human (His)

Sulfotransferase 1B1 (SULT1B1) is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of dopamine, small phenols such as 1-naphthol and p-nitrophenol, thyroid hormones and many xenobiotic compounds.SULT1B1 may play a role in gut microbiota-host metabolic interaction.SULT1B1 Protein, Human (His) is the recombinant human-derived SULT1B1 protein, expressed by E.coli , with N-6*His labeled tag.

Sulfotransferase 1B1 (SULT1B1) is a sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine (T3) and reverse triiodothyronine (rT3).
SULT1B1 may play a role in gut microbiota-host metabolic interaction, as one of the products derived from a dietary tyrosine-derived metabolite produced by gut bacteria, 4-EPS, crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system^[1][2][3][4].

Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is 37.98 pmol/min/μg that incubate at 37°C for 20 minutes.

Human

E. coli

N-6*His

AAH10895.1 (M1-I296)

27284  [NCBI]

MLSPKDILRKDLKLVHGYPMTCAFASNWEKIEQFHSRPDDIVIATYPKSGTTWVSEIIDMILNDGDIEKCKRGFITEKVPMLEMTLPGLRTSGIEQLEKNPSPRIVKTHLPTDLLPKSFWENNCKMIYLARNAKDVSVSYYHFDLMNNLQPFPGTWEEYLEKFLTGKVAYGSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIRFLEKNLNDEILDRIIHHTSFEVMKDNPLVNYTHLPTTVMDHSKSPFMRKGTAGDWKNYFTVAQNEKFDAIYETEMSKTALQFRTEI

28-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.