T4 DNA Polymerase Protein, T4 phage (His)

T4 DNA Polymerase, a pivotal enzyme in viral DNA replication, performs dual functions crucial for genome synthesis. As a polymerase, it synthesizes DNA, and with its exonucleolytic activity, it proofreads by degrading single-stranded DNA in the 3'- to 5'-direction, ensuring the accuracy and fidelity of replicated viral genomic DNA. T4 DNA Polymerase Protein, T4 phage (His) is the recombinant T4 DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. T4 DNA Polymerase Protein, T4 phage (His), has molecular weight of ~103.6 kDa.

T4 DNA Polymerase, a key enzyme in viral DNA replication, exhibits dual enzymatic functions critical for its role in genome synthesis. This polymerase showcases both DNA synthesis, where it acts as a polymerase, and exonucleolytic activity, facilitating the degradation of single-stranded DNA in the 3'- to 5'-direction. The latter function serves a proofreading purpose, ensuring the accuracy and fidelity of the replicated viral genomic DNA.

The specific activity is ≥3000 U/mL.Unit Definition:One unit is defined as the amount of enzyme that catalyzes the incorporation of deoxyribonucleotids(dNTPs) into polynucleotides at 37 ºC for 30 minutes. Reaction Buffer: 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl, PH 7.9 at 25℃.

Others

E. coli

N-6*His

P04415

1258685  [NCBI]

MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNDEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDHSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG

Approximately 103.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 100 mM KPO4 , 1 mM DTT , 50 % Glycerol, (pH 6.5 @ 25°C)

N/A.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.