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TCEB3 Protein, Human (Sf9)

Cat. No.: HY-P701515
Handling Instructions

TCEB3, also known as SIII, acts as a key transcription elongation factor that promotes RNA polymerase II progression beyond the blocking site. It interacts with SIII regulatory subunits B and C to form the elongin BC complex, which significantly enhances the transcriptional activity of subunit A. TCEB3 Protein, Human (Sf9) is the recombinant human-derived TCEB3 protein, expressed by Sf9 insect cells , with tag free. The total length of TCEB3 Protein, Human (Sf9) is 797 a.a., .

For research use only. We do not sell to patients.

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Description

TCEB3, also known as SIII, acts as a key transcription elongation factor that promotes RNA polymerase II progression beyond the blocking site. It interacts with SIII regulatory subunits B and C to form the elongin BC complex, which significantly enhances the transcriptional activity of subunit A. TCEB3 Protein, Human (Sf9) is the recombinant human-derived TCEB3 protein, expressed by Sf9 insect cells , with tag free. The total length of TCEB3 Protein, Human (Sf9) is 797 a.a., .

Background

TCEB3, also referred to as SIII, functions as a general transcription elongation factor, facilitating RNA polymerase II transcription elongation beyond template-encoded arresting sites. The transcriptional activity of subunit A is significantly boosted by its interaction with the dimeric complex formed by the SIII regulatory subunits B and C, collectively known as the elongin BC complex. TCEB3 operates within a multisubunit assembly, including elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1, and CUL5. In this context, it polyubiquitinates monoubiquitinated POLR2A, contributing to the regulation of transcriptional processes.

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

Q14241 (H2-R798)

Gene ID

6924

Molecular Construction
N-term
TCEB3 (H2-R798)
Accession # Q14241
C-term
Synonyms
ELOA (TCEB3); Elongin-A; EloA; Elongin 110 kDa subunit; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Transcription elongation factor B polypeptide 3
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

TCEB3 Protein, Human (Sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TCEB3 Protein, Human (Sf9)
Cat. No.:
HY-P701515
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