TCN1 Protein, Human (P.pastoris, His)

The TCN1 protein is critical for mitochondrial processes and binds to the mitochondrial light chain promoter to regulate mitochondrial transcription. As a key component of the mitochondrial transcription initiation complex (including TFB2M and POLRMT), TCN1 plays a crucial role in the basal transcription of mitochondrial DNA. TCN1 Protein, Human (P.pastoris, His) is the recombinant human-derived TCN1 protein, expressed by P. pastoris , with N-His labeled tag. The total length of TCN1 Protein, Human (P.pastoris, His) is 410 a.a., with molecular weight of ~47.6 kDa.

TFAM, a key participant in mitochondrial processes, exhibits the ability to bind to the mitochondrial light strand promoter, thereby contributing to the regulation of mitochondrial transcription. As an integral component of the mitochondrial transcription initiation complex, comprising TFB2M, TFAM, and POLRMT, it plays a crucial role in the basal transcription of mitochondrial DNA. Within this complex, TFAM not only recruits POLRMT to specific promoters but also collaborates with TFB2M to induce structural changes in POLRMT, facilitating promoter opening and trapping of the DNA non-template strand. Essential for accurate promoter recognition by mitochondrial RNA polymerase, TFAM promotes transcription initiation from select promoters. Additionally, TFAM showcases DNA-unwinding capabilities and imparts a U-turn shape to the mitochondrial light strand promoter DNA through its HMG boxes. Beyond its transcriptional functions, TFAM is vital for maintaining normal mitochondrial DNA levels and may play a role in organizing and compacting both mitochondrial and nuclear DNA, particularly during spermatogenesis.

Human

P. pastoris

N-His

P20061 (24E-433Y)

6947  [NCBI]

EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY

Approximately 47.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.