TCPTP Protein, Human (sf9)

TCPTP is a non-receptor tyrosine-specific phosphatase that critically regulates various kinases (INSR, EGFR, JAK, Src) affecting hematopoiesis, inflammation, cell proliferation, and glucose homeostasis. In the immune system, TCPTP regulates T cell receptor signaling and inhibits cytokine pathways (IL2, interferon). TCPTP Protein, Human (sf9) is the recombinant human-derived TCPTP protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes), , , , mutation.

TCPTP, a non-receptor type tyrosine-specific phosphatase, acts as a crucial regulator by dephosphorylating various receptor and non-receptor protein tyrosine kinases, including INSR, EGFR, CSF1R, PDGFR, JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3, and STAT6, either in the nucleus or the cytoplasm. This phosphatase exerts negative control over diverse signaling pathways and biological processes such as hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. In the immune system, TCPTP plays a multifaceted role, influencing T-cell receptor signaling by dephosphorylating FYN and LCK to regulate T-cell differentiation and activation. Additionally, it negatively modulates cytokine-mediated signaling, including IL2, interferon, IL6, and IL4 pathways. Beyond immune regulation, TCPTP participates in anchorage-dependent, negative regulation of EGF-stimulated cell growth and PDGF receptor-beta signaling, thus influencing cell proliferation. Moreover, it plays a vital role in glucose homeostasis, negatively regulating the insulin receptor signaling pathway and controlling gluconeogenesis and liver glucose production through the IL6 signaling pathway. TCPTP's broad impact underscores its significance in diverse cellular processes, and it may also have DNA binding capabilities.

Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate.The specific activity is > 30 nmoles/min/μg.

Human

Sf9 insect cells

Tag Free

P17706-1 (N-G&P, M1-N314)

5771  [NCBI]

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0 or 20 mM Tris, pH 8.0, 300 mM NaCl, 10% Glycerol.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.