TFF3 Protein, Human (HEK293, His)

Trefoil factor 3 (TFF3) is a small peptide (59 amino acids; 7 kDa) that is a member of the trefoil family and a stable secretory protein expressed in gastrointestinal mucosa, playing a role in restitution and regeneration of epithelia. The mode of action of Tff3 ranges from a simple increase in mucous viscosity, cytoprotection, and antiapoptotic and chemotactic effects to more complex immune regulatory functions, and has a strong association with tumorigenesis. TFF3 Protein, Human (HEK293, His) is the recombinant human-derived TFF3 protein, expressed by HEK293 , with C-His labeled tag. The total length of TFF3 Protein, Human (HEK293, His) is 59 a.a., with molecular weight of ~8 kDa.

Trefoil factor 3 (TFF3) is a small peptide (59 amino acids; 7 kDa) that is a member of the trefoil family which are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides with three-loop structure. TFF3 is a stable secretory protein expressed in gastrointestinal mucosa, playing a role in restitution (cell migration to heal superficial lesions) and regeneration (differentiation and proliferation as repair) of epithelia, being involved in the maintenance and repair of the intestinal mucosa and promotes the mobility of epithelial cells in healing processes. The mode of action of Tff3 ranges from a simple increase in mucous viscosity, cytoprotection, and antiapoptotic and chemotactic effects to more complex immune regulatory functions. Tff expression patterns are altered in various tumors, implying a strong association with tumorigenesis. Tff3 presents in the bloodstream and various other organs, including the liver, brain, pancreas, and lymphoid tissue^[1][2][3].

Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF3 can not effectively induce ERK1/2 phosphorylation.

Human

HEK293

C-His

Q07654 (E22-F80)

7033  [NCBI]

Approximately 8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.