TFIIB Protein, Human (GST)

TFIIB protein is a general transcription factor involved in the initiation of RNA polymerase II. TFIIB Protein, Human (GST) is the recombinant human-derived TFIIB protein, expressed by E. coli , with N-GST labeled tag. The total length of TFIIB Protein, Human (GST) is 316 a.a., with molecular weight of ~62.0 kDa.

The TFIIB Protein is a key general transcription factor involved in the initiation of RNA polymerase II (Pol II)-mediated transcription. It plays a crucial role in pre-initiation complex (PIC) formation and recruits Pol II to the promoter DNA. Together with the TATA box-bound TBP, TFIIB forms the core initiation complex, acting as a bridge between TBP and the Pol II-TFIIF complex. Notably, it is released from the PIC early during the initiation and elongation transition of transcription, reassociating with TBP in the subsequent transcription cycle. TFIIB associates with chromatin at core promoter-specific regions and binds to two distinct DNA core promoter consensus sequence elements known as IIB-recognition elements (BREs). These BREs, located immediately upstream (BREu) and downstream (BREd) of the TATA box element, are recognized in a TBP-independent manner. Additionally, TFIIB exhibits autoacetyltransferase activity, contributing to activated transcription and modulating transcription start site selection. The multifaceted functions of TFIIB highlight its central role in the intricate process of transcription initiation.

Human

E. coli

N-GST

Q00403 (M1-L316)

2959  [NCBI]

MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL

Approximately 62.0 kDa

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.