TFRC Protein, Cynomolgus (HEK293, His)

TFRC is a key protein in cellular iron uptake, coordinating receptor-mediated endocytosis of the ligand transferrin into specialized endosomes, where endosomal acidification triggers iron release. TFRC actively regulates T and B cell proliferation through iron uptake. TFRC Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TFRC protein, expressed by HEK293 , with N-His labeled tag. The total length of TFRC Protein, Cynomolgus (HEK293, His) is 672 a.a., with molecular weight of 78-80 kDa.

TFRC, a pivotal protein in cellular iron uptake, orchestrates receptor-mediated endocytosis of ligand-occupied transferrin into specialized endosomes, where endosomal acidification triggers iron release. The apotransferrin-receptor complex undergoes recycling to the cell surface while a return to neutral pH, leading to the loss of apotransferrin affinity for its receptor. Essential for erythrocyte and nervous system development, TFRC positively regulates T and B cell proliferation through iron uptake. Acting as a lipid sensor, TFRC modulates mitochondrial fusion by regulating the JNK pathway. Low dietary stearate levels induce JNK pathway activation, resulting in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2, inhibiting mitochondrial fusion. Conversely, high stearate levels stearoylate TFRC, suppressing JNK pathway activation and preventing MFN2 degradation. TFRC also can function as a homodimer with disulfide linkage, binding one transferrin molecule per subunit, and interacts with SH3BP4 and STEAP3, the latter facilitating TFRC endocytosis in erythroid precursor cells.

Measured by its ability to neutralize transferrin-mediated proliferation of MCF-7 cells. The ED₅₀ for this effect is typically 0.2-2 μg/mL.

Cynomolgus

HEK293

N-His

XP_005545315 (C89-F760)

102136007  [NCBI]

78-80 kDa

Greater than 90% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.