TGM2/Transglutaminase 2 Protein, Human (sf9, His, solution)

In summary, TGM2 is a multifunctional enzyme that plays crucial roles in various cellular processes. It acts as a protein-glutamine gamma-glutamyl transferase, mediating the cross-linking of proteins involved in cellular functions. TGM2/Transglutaminase 2 Protein, Human (sf9, His, solution) is the recombinant human-derived TGM2/Transglutaminase 2, expressed by Sf9 insect cells , with N-His labeled tag. ,

Transglutaminase 2 (TGM2) is a calcium-dependent acyltransferase that facilitates the formation of covalent bonds between peptide-bound glutamine and various primary amines, leading to the cross-linking or amination of proteins. It plays a crucial role in diverse biological processes, including bone development, angiogenesis, wound healing, cellular differentiation, chromatin modification, and apoptosis. TGM2 acts as a protein-glutamine gamma-glutamyltransferase by mediating the cross-linking of various proteins involved in essential cellular functions. Under physiological conditions, its protein cross-linking activity is inhibited by GTP, with relief of inhibition in response to Ca(2+) during stress. When secreted, TGM2 catalyzes the cross-linking of extracellular matrix proteins, forming stable scaffolds. In apoptosis, TGM2 contributes to the condensation of the cytoplasm by promoting the cross-linking of cytoskeletal proteins and mediates the irreversible formation of scaffolds that stabilize dying cells before clearance by phagocytosis, preventing the leakage of harmful intracellular components. Additionally, TGM2 performs diverse protein post-translational modifications, including aminylation of serotonin, dopamine, noradrenaline, or histamine into glutamine residues, generating protein serotonylation, dopaminylation, noradrenalinylation, or histaminylation, respectively. It plays a key role in chromatin organization by mediating serotonylation and dopaminylation of histone H3. Moreover, TGM2 acts as a mediator of neurotransmission-independent roles of nuclear dopamine in ventral tegmental area (VTA) neurons, regulating relapse-related transcriptional plasticity in the reward system. It also regulates vein remodeling by mediating serotonylation and subsequent inactivation of ATP2A2/SERCA2. Furthermore, TGM2 acts as a protein deamidase, converting specific glutamine residues to glutamate, and may function as an isopeptidase cleaving previously formed cross-links. It can also participate in signaling pathways independently of its acyltransferase activity, acting as a signal transducer in alpha-1 adrenergic receptor-mediated stimulation of phospholipase C-delta (PLCD) activity, and is required for coupling alpha-1 adrenergic agonists to the stimulation of phosphoinositide lipid metabolism. TGM2 exhibits cytotoxic activity, inducing apoptosis independently of its acyltransferase activity.

Human

Sf9 insect cells

N-His

P21980-1 (M1-A687)

7052

Approximately 80 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, pH 7.5, 10% glycerol, 0.5 mM TCEP.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.