Thymidylate Protein, Human (HEK293, His)

Thymidylate proteins play a key role in DNA synthesis by catalyzing the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This enzymatic process is critical for the production of dTDP, the direct precursor of the DNA building block dTTP. Thymidylate Protein, Human (HEK293, His) is the recombinant human-derived Thymidylate protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Thymidylate Protein, Human (HEK293, His) is 212 a.a., with molecular weight of ~26.0 kDa.

Thymidylate kinase, an essential enzyme in nucleotide metabolism, catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). This phosphorylation reaction is a crucial step in the biosynthesis of deoxythymidine triphosphate (dTTP), a building block for DNA replication and repair. Thymidylate kinase utilizes ATP as the preferred phosphoryl donor, and the reaction is facilitated in the presence of Mg(2+). The enzyme's activity is integral to the maintenance of balanced nucleotide pools and the precise regulation of DNA synthesis in cells. Understanding the functions of thymidylate kinase is essential for unraveling the intricate processes involved in DNA replication and cellular proliferation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P23919 (M1-K212)

1841  [NCBI]

MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK

Approximately 26.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 5% Sucrose, 0.05% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.