TIE-2 Protein, Rat (HEK293, Fc)

TIE-2 protein has growth factor binding activity and is involved in ossification, cAMP response, and estrogen response. It is predicted to be present in a variety of cellular components, as a component of the plasma membrane, and as part of receptor complexes. TIE-2 Protein, Rat (HEK293, Fc) is the recombinant rat-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Rat (HEK293, Fc) is 721 a.a., with molecular weight of 120-130 kDa.

TIE-2 Protein possesses growth factor binding activity and is involved in various processes, including endochondral ossification, response to cAMP, and response to estrogen. It is predicted to be located in diverse cellular components such as basolateral plasma membrane, centriolar satellite, and microvillus, and is expected to be an integral component of the plasma membrane and part of a receptor complex. The predicted colocalization with actin filament and stress fiber suggests its potential involvement in cytoskeletal dynamics. Notably, TIE-2 serves as a biomarker for several diseases, including acute kidney tubular necrosis, brain ischemia, gastric ulcer, goiter, and hepatocellular carcinoma. The human ortholog(s) of this gene have been implicated in arteriovenous malformation and multiple cutaneous and mucosal venous malformations. The expression of TIE-2 is biased, with prominent levels observed in lung (RPKM 772.5), heart (RPKM 237.7), and various other tissues.

Immobilized Rat Tie-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Angiopoietin-2. The ED₅₀ for this effect is 22.89 ng/mL.

Rat

HEK293

C-hFc

NP_001099207.1 (A23-L743)

89804  [NCBI]

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKML

Approximately 115-130 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.