TIM Protein, Human (His)

TIM protein is a highly efficient metabolic enzyme that catalyzes the rapid interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) during glycolysis and gluconeogenesis. Its key role in glucose metabolism is complemented by an additional responsibility: the production of methylglyoxal, a reactive cytotoxic by-product. TIM Protein, Human (His) is the recombinant human-derived TIM protein, expressed by E. coli , with N-6*His labeled tag. The total length of TIM Protein, Human (His) is 249 a.a., with molecular weight of ~30.0 kDa.

Triosephosphate isomerase (TIM) stands out as an exceptionally efficient metabolic enzyme crucial for catalyzing the rapid interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in the central pathways of glycolysis and gluconeogenesis. Beyond its pivotal role in glucose metabolism, TIM assumes an additional responsibility in the production of methylglyoxal, a reactive and cytotoxic side-product. Methylglyoxal exhibits the potential to modify and induce alterations in proteins, DNA, and lipids, emphasizing the dual nature of TIM's involvement in fundamental metabolic processes and the generation of cytotoxic byproducts.

Human

E. coli

N-6*His

P60174 (M1-Q249)

7167  [NCBI]

MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

Approximately 30.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.