TMK Protein, Bacillus anthracis (His)

TMK proteins play a central role by catalyzing the phosphorylation of deoxythymidine monophosphate (dTMP), a key step in the synthesis of deoxythymidine triphosphate (dTTP). This process converts dTMP into deoxythymidine diphosphate (dTDP), an important intermediate in dTTP biosynthesis. TMK Protein, Bacillus anthracis (His) is the recombinant TMK protein, expressed by E. coli , with N-6*His labeled tag. The total length of TMK Protein, Bacillus anthracis (His) is 208 a.a., with molecular weight of ~25 kDa.

The TMK Protein orchestrates a crucial role in catalyzing the phosphorylation of deoxythymidine monophosphate (dTMP), a pivotal step in the synthesis of deoxythymidine triphosphate (dTTP) within both the de novo and salvage pathways. This enzymatic process leads to the conversion of dTMP into deoxythymidine diphosphate (dTDP), serving as a vital intermediate in dTTP biosynthesis. The involvement of TMK Protein in facilitating this phosphorylation event underscores its central regulatory role in modulating the production of dTTP, a fundamental nucleotide essential for DNA synthesis. A comprehensive understanding of TMK Protein's engagement in these pathways enhances our insight into cellular nucleotide dynamics and, consequently, contributes to the maintenance of genomic stability.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

E. coli

N-6*His

C3LJ02 (M1-L208)

45020070  [NCBI]

MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL

Approximately 25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.2 μm filtered solution of 50 mM PBS, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.