TMP21/p23 Protein, Human (HEK293, Fc)

TMP21/p23 proteins act as cargo receptors in endoplasmic reticulum (ER) and Golgi vesicle trafficking, mediating quality control. TMP21/p23 Protein, Human (HEK293, Fc) is the recombinant human-derived TMP21/p23 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of TMP21/p23 Protein, Human (HEK293, Fc) is 154 a.a., with molecular weight of ~44 KDa.

TMP21/p23, a cargo receptor integral to protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi, plays a crucial role in coordinating vesicle coat formation and cargo transport. It functions primarily in the early secretory pathway, cycling between the ER, ER-Golgi intermediate compartment (ERGIC), and Golgi. Notably, TMP21/p23 acts in both anterograde and retrograde vesicle-mediated transport, participating in the transport of GPI-anchored proteins and influencing the biogenesis of COPI vesicles. Beyond its cargo receptor activity, TMP21/p23 may also function as a protein channel, facilitating the translocation of leaderless cytoplasmic cargo into the ERGIC. Additionally, it is involved in regulating the trafficking of various proteins, including G protein-coupled receptors, contributing to receptor resensitization. TMP21/p23 forms complexes with other p24 family members and interacts with multiple components of the vesicle coat machinery, highlighting its multifaceted role in intracellular protein transport.

Human

HEK293

C-mFc

P49755/NP_006818.3 (I32-R185)

10972  [NCBI]

ISFHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTR

Approximately 45-50 kDa.

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.