TNIK Protein, Human (Sf9)

TNIK protein is a key activator of the Wnt signaling pathway and regulates gene expression by phosphorylating TCF4/TCF7L2 at the promoter of Wnt target genes. TNIK is located upstream of the JUN N-terminal pathway and contributes to the complex Wnt signaling cascade. TNIK Protein, Human (Sf9) is the recombinant human-derived TNIK protein, expressed by Sf9 insect cells , with tag free. The total length of TNIK Protein, Human (Sf9) is 304 a.a., .

TNIK, a serine/threonine kinase, emerges as a pivotal activator of the Wnt signaling pathway, playing a crucial role in gene expression regulation. Its involvement extends to the phosphorylation of TCF4/TCF7L2 at promoters of Wnt target genes, thereby facilitating their activation. Positioned upstream of the JUN N-terminal pathway, TNIK contributes to the intricate cascade of Wnt signaling. Beyond its canonical functions, TNIK is implicated in the response to environmental stress and forms part of a signaling complex, comprising NEDD4, RAP2A, and TNIK, which orchestrates neuronal dendrite extension and arborization during development. In a broader context, TNIK exhibits potential roles in cytoskeletal rearrangements and the regulation of cell spreading. Notably, it exerts its influence by phosphorylating SMAD1 at Thr-322, thereby expanding its regulatory repertoire in diverse cellular processes.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.

Human

Sf9 insect cells

Tag Free

Q9UKE5 (D11-G314)

23043

34.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 10% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.