TPK1 Protein, Human (HEK293, His)

Thiamin pyrophosphokinase 1 (TPK1) functions as a homodimer and catalyzes the conversion of thiamine (Vitamin B1) to thiamine pyrophosphate (TDP), a cofactor and a transient intermediate carrier of the aldehyde group for some enzymes of the glycolytic and energy production pathways. TPK1 can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. TPK1 Protein, Human (HEK293, His) is the recombinant human-derived TPK1 protein, expressed by HEK293 , with C-6*His labeled tag.

Thiamin pyrophosphokinase 1 (TPK1) functions as a homodimer and catalyzes the conversion of thiamine (Vitamin B1) to thiamine pyrophosphate (TDP), a cofactor for some enzymes of the glycolytic and energy production pathways. TDP serves as a transient intermediate carrier of the aldehyde group in the reactions of TDP-dependent enzymes such as pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, transketolase, and others. TPK1 can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Defects in TPK1 gene are a cause of thiamine metabolism dysfunction syndrome-5^[1].

Human

HEK293

C-6*His

AAH68460.1 (M1-S243)

27010  [NCBI]

MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCSQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.