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TPM2 Protein, Human (His)

Cat. No.: HY-P72264
COA Handling Instructions

The TPM2 protein binds actin filaments in muscle and nonmuscle cells and centrally regulates calcium-dependent striated muscle contraction in vertebrates, particularly in concert with the troponin complex. In smooth muscle cells, TPM2 helps regulate contraction by interacting with calcium-binding proteins. TPM2 Protein, Human (His) is the recombinant human-derived TPM2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TPM2 Protein, Human (His) is 271 a.a., with molecular weight of ~38 kDa.

For research use only. We do not sell to patients.

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Description

The TPM2 protein binds actin filaments in muscle and nonmuscle cells and centrally regulates calcium-dependent striated muscle contraction in vertebrates, particularly in concert with the troponin complex. In smooth muscle cells, TPM2 helps regulate contraction by interacting with calcium-binding proteins. TPM2 Protein, Human (His) is the recombinant human-derived TPM2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TPM2 Protein, Human (His) is 271 a.a., with molecular weight of ~38 kDa.

Background

TPM2 protein exhibits specific binding to actin filaments in both muscle and non-muscle cells, playing a central role in the calcium-dependent regulation of vertebrate striated muscle contraction, particularly in association with the troponin complex. In smooth muscle cells, TPM2 contributes to the regulation of contraction through its interaction with caldesmon. Moreover, in non-muscle cells, TPM2 is implicated in the stabilization of actin filaments within the cytoskeleton, indicating its versatile functions across cellular contexts. The non-muscle isoform of TPM2 may additionally participate in agonist-mediated receptor internalization. Structurally, TPM2 forms homodimers and heterodimers, the latter composed of an alpha chain (TPM1, TPM3, or TPM4) and a beta chain (TPM2), highlighting its diverse molecular associations and underscoring its pivotal roles in both muscle contraction and cellular structural dynamics.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P07951 (D14-L284)

Gene ID
Molecular Construction
N-term
6*His
TPM2 (D14-L284)
Accession # P07951
C-term
Synonyms
AMCD1; TMSA; TMSB; TPM2; TRK; Tropomyosin beta chain
AA Sequence

DKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESKCGDLEEELKIVTNNLKSLEAQADKYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEDEVYAQKMKYKAISEELDNALNDITSL

Molecular Weight

Approximately 38 kDa

Purity
  • Greater than 92% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS , 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TPM2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TPM2 Protein, Human (His)
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