TPM3 Protein, Human

TPM3 is a member of the tropomyosin family that binds actin filaments in muscle and nonmuscle cells. In striated muscle, it regulates calcium-dependent contraction together with the troponin complex. TPM3 Protein, Human is the recombinant human-derived TPM3 protein, expressed by E. coli , with tag free. The total length of TPM3 Protein, Human is 248 a.a., with molecular weight of ~20.0 kDa.

The TPM3 protein, a member of the tropomyosin family, exhibits versatile functions in binding to actin filaments both in muscle and non-muscle cells. In conjunction with the troponin complex, TPM3 plays a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. Its involvement in smooth muscle contraction is mediated through interaction with caldesmon. Beyond muscle-related functions, TPM3 is implicated in stabilizing actin filaments within the cytoskeleton of non-muscle cells. Structurally, TPM3 forms homodimers and heterodimers, combining with alpha (TPM1, TPM3, or TPM4) and beta (TPM2) chains. Additionally, TPM3 interacts with TMOD1, further contributing to its multifaceted role in modulating actin dynamics across various cellular contexts.

Human

E. coli

Tag Free

P06753-2 (M1-M248)

7170  [NCBI]

MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQTLLDLNEM

Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.