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TPST1 Protein, Human (HEK293, His)

Cat. No.: HY-P77255
SDS COA Handling Instructions

TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293 , with N-His labeled tag. ,

For research use only. We do not sell to patients.

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Description

TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293 , with N-His labeled tag. ,

Background

Tyrosylprotein sulfotransferase 1 (TPST1) is a key enzyme that catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, utilizing 3'-phosphoadenylyl sulfate (PAPS) as a cosubstrate. This enzymatic process involves the transfer of a sulfate group to tyrosine residues, modulating the structure and function of proteins with acidic motifs. TPST1-mediated tyrosine sulfation is crucial for the post-translational modification of proteins involved in various biological processes, including cell signaling and receptor activation. By participating in the sulfation of tyrosine residues, TPST1 contributes to the diversity of protein modifications, influencing the interactions and activities of sulfated proteins in cellular pathways. This enzyme's role in tyrosine sulfation underscores its importance in the regulation of protein function and cellular signaling events (adapted from the provided passage).

Species

Human

Source

HEK293

Tag

N-His

Accession

O60507 (Q26-E370)

Gene ID
Molecular Construction
N-term
His
TPST1 (Q26-E370)
Accession # O60507
C-term
Synonyms
Protein-tyrosine sulfotransferase 1; Tyrosylprotein sulfotransferase 1; TPST-1
AA Sequence

QHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE

Molecular Weight

Approximately 43-57 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TPST1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TPST1 Protein, Human (HEK293, His)
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HY-P77255
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