TRAILR4/TNFRSF10D Protein, Human (Biotinylated, HEK293, His-Avi)

TRAILR4/TNFRSF10D protein is a receptor for TRAIL and lacks the ability to induce apoptosis due to the truncated death domain. Paradoxically, not only does it fail to induce apoptosis, but it also prevents TRAIL-mediated apoptosis. TRAILR4/TNFRSF10D Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TRAILR4/TNFRSF10D protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of TRAILR4/TNFRSF10D Protein, Human (Biotinylated, HEK293, His-Avi) is 156 a.a., with molecular weight of 38-50 kDa.

The TRAILR4/TNFRSF10D Protein functions as a receptor for the cytotoxic ligand TRAIL, although it contains a truncated death domain, rendering it incapable of inducing apoptosis. Paradoxically, TRAILR4/TNFRSF10D not only fails to induce apoptosis but also serves a protective role against TRAIL-mediated apoptosis. There is conflicting information regarding its ability to activate the NF-kappa-B pathway, with some studies suggesting that it cannot induce this pathway, while others propose that it has the capability to activate NF-kappa-B. The dual nature of TRAILR4/TNFRSF10D in interacting with TRAIL, both as a receptor and as a protective factor against apoptosis, underscores the complexity of its regulatory functions in cellular responses to TRAIL signaling.

Measured by its binding ability in a functional ELISA. When immobilized Human TRAIL at 2 μg/mL (100μl/Well), can bind Human TRAIL at 2 μg/mL and the EC₅₀ is 0.16 ug/ml.

Human

HEK293

C-Avi;C-His

Q9UBN6 (A56-H211)

8793  [NCBI]

38-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.