Transferrin Protein, Mouse (HEK293, His)

Transferrin is an important iron-binding transport protein that combines two Fe(3+) ions with an anion, usually bicarbonate.Its main function is to transport iron from the site of absorption to the site of storage and utilization.Transferrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Transferrin protein, expressed by HEK293 , with C-10*His labeled tag.

Transferrin, a pivotal iron-binding transport protein, exhibits the capacity to bind two Fe(3+) ions in conjunction with an anion, typically bicarbonate. Its primary responsibility lies in facilitating the transport of iron from absorption sites and heme degradation to locations designated for storage and utilization within the body. Beyond its fundamental role in iron homeostasis, serum transferrin may additionally play a role in promoting cell proliferation. Structured as a monomer, transferrin's multifaceted functions underscore its significance in orchestrating the intricate balance of iron distribution and cellular processes essential for overall physiological well-being.

Measured by its ability to inhibit proliferation of A549 cells. The ED₅₀ for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.

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  Measured by its ability to inhibit proliferation of A549 cells. The ED₅₀ for this effect is 187.3 ng/mL, corresponding to a specific activity is 5339.028 U/mg.

Mouse

HEK293

C-10*His

Q921I1 (V20-H697)

22041  [NCBI]

VPDKTVKWCAVSEHENTKCISFRDHMKTVLPPDGPRLACVKKTSYPDCIKAISASEADAMTLDGGWVYDAGLTPNNLKPVAAEFYGSVEHPQTYYYAVAVVKKGTDFQLNQLEGKKSCHTGLGRSAGWVIPIGLLFCKLSEPRSPLEKAVSSFFSGSCVPCADPVAFPKLCQLCPGCGCSSTQPFFGYVGAFKCLKDGGGDVAFVKHTTIFEVLPEKADRDQYELLCLDNTRKPVDQYEDCYLARIPSHAVVARKNNGKEDLIWEILKVAQEHFGKGKSKDFQLFSSPLGKDLLFKDSAFGLLRVPPRMDYRLYLGHNYVTAIRNQQEGVCPEGSIDNSPVKWCALSHLERTKCDEWSIISEGKIECESAETTEDCIEKIVNGEADAMTLDGGHAYIAGQCGLVPVMAEYYESSNCAIPSQQGIFPKGYYAVAVVKASDTSITWNNLKGKKSCHTGVDRTAGWNIPMGMLYNRINHCKFDEFFSQGCAPGYEKNSTLCDLCIGPLKCAPNNKEEYNGYTGAFRCLVEKGDVAFVKHQTVLDNTEGKNPAEWAKNLKQEDFELLCPDGTRKPVKDFASCHLAQAPNHVVVSRKEKAARVKAVLTSQETLFGGSDCTGNFCLFKSTTKDLLFRDDTKCFVKLPEGTTPEKYLGAEYMQSVGNMRKCSTSRLLEACTFHKH

approximately 85 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.