Transferrin Protein, Pig (HEK293, His)

Transferrin is an important iron-binding transport protein that binds two Fe(3+) ions, usually with anions such as bicarbonate. Crucially, it transports iron from the site of absorption and heme degradation to the site of storage and utilization. Transferrin Protein, Pig (HEK293, His) is the recombinant pig-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Transferrin Protein, Pig (HEK293, His) is 694 a.a., with molecular weight of ~80 kDa.

Transferrins, as iron-binding transport proteins, exhibit the capability to bind two Fe(3+) ions, often in conjunction with the binding of an anion, typically bicarbonate. Functionally, transferrin plays a crucial role in transporting iron from sites of absorption and heme degradation to locations dedicated to storage and utilization within the body. Beyond its primary involvement in iron homeostasis, serum transferrin may play an additional role in stimulating cell proliferation. Structured as a monomer, transferrin's versatility underscores its significance in orchestrating the intricate processes of iron transport and cellular regulation, contributing to essential physiological functions within the organism.

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×10⁴ units/mg.

• 
  Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×10⁴ units/mg.

Pig

HEK293

C-His

B3CL06 (Q22-T715)

396996  [NCBI]

QKTVRWCTISNQEANKCSSFRENMSKAVKNGPLVSCVKKSSYLDCIKAIRDKEADAVTLDAGLVFEAGLAPYNLKPVVAEFYGQKDNPQTHYYAVAVVKKGSNFQWNQLQGKRSCHTGLGRSAGWIIPMGLLYDQLPEPRKPIEKAVASFFSSSCVPCADPVNFPKLCQQCAGKGAEKCACSNHEPYFGYAGAFNCLKEDAGDVAFVKHSTVLENLPDKADRDQYELLCRDNTRRPVDDYENCYLAQVPSHAVVARSVDGQEDSIWELLNQAQEHFGRDKSPDFQLFSSSHGKDLLFKDSANGFLRIPSKMDSSLYLGYQYVTALRNLREEISPDSSKNECKKVRWCAIGHEETQKCDAWSINSGGKIECVSAENTEDCIAKIVKGEADAMSLDGGYIYIAGKCGLVPVLAENYKTEGENCVNTPEKGYLAVAVVKKSSGPDLNWNNLKGKKSCHTAVDRTAGWNIPMGLLYNKINSCKFDQFFGEGCAPGSQRNSSLCALCIGSERAPGRECLANNHERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREPVDNAENCHLARAPNHAVVARDDKVTCVAEELLKQQAQFGRHVTDCSSSFCMFKSNTKDLLFRDDTQCLARVGKTTYESYLGADYITAVANLRKCSTSKLLEACTFHSAKNPRVETTT

Approximately 80 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.