Transglutaminase 3/TGM3 Protein, Human (sf9, His)

Transglutaminase 3 (TGM3) serves as a catalyst in the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in diverse proteins, alongside mediating the conjugation of polyamines to proteins. This enzymatic activity plays a crucial role in the intricate process of cornified envelope (CE) formation, a specialized structure comprising covalent cross-links of proteins beneath the plasma membrane in fully differentiated keratinocytes. TGM3 is actively involved in cross-linking small proline-rich proteins (SPRR1 and SPRR2) and loricrin (LOR) to create small interchain oligomers. These oligomers are subsequently cross-linked by TGM1 onto the developing CE scaffold, contributing to the structural integrity of this vital component. Notably, TGM3's involvement extends to hair follicles, where it participates in cross-linking structural proteins to fortify the inner root sheath. Transglutaminase 3/TGM3 Protein, Human (sf9, His) is the recombinant human-derived Transglutaminase 3/TGM3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Transglutaminase 3/TGM3 Protein, Human (sf9, His) is 692 a.a., with molecular weight of ~70 kDa.

Transglutaminase 3 (TGM3) serves as a catalyst in the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in diverse proteins, alongside mediating the conjugation of polyamines to proteins. This enzymatic activity plays a crucial role in the intricate process of cornified envelope (CE) formation, a specialized structure comprising covalent cross-links of proteins beneath the plasma membrane in fully differentiated keratinocytes. TGM3 is actively involved in cross-linking small proline-rich proteins (SPRR1 and SPRR2) and loricrin (LOR) to create small interchain oligomers. These oligomers are subsequently cross-linked by TGM1 onto the developing CE scaffold, contributing to the structural integrity of this vital component. Notably, TGM3's involvement extends to hair follicles, where it participates in cross-linking structural proteins to fortify the inner root sheath.

Measured by its ability to cleave a synthetic peptide Benzvloxvcarbonvl-GIn-Glvand NH2OH.The specific activity is 1018.3 pmol/min/μg, as measured under the described conditions.

Human

Sf9 insect cells

N-His

Q08188 (A2-E693)

7053  [NCBI]

Approximately 78 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% gly

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.