TrkC Protein, Human (HEK293, His)

TrkC Protein, a receptor tyrosine kinase, crucially contributes to nervous system and potential heart development. Upon NTF3/neurotrophin-3 binding, TrkC undergoes autophosphorylation, activating pathways like phosphatidylinositol 3-kinase/AKT and MAPK. These pathways regulate cell survival and differentiation, emphasizing TrkC's pivotal role in essential cellular processes during development. TrkC Protein, Human (HEK293, His) is the recombinant human-derived TrkC protein, expressed by HEK293 , with C-His labeled tag.

TrkC Protein, a receptor tyrosine kinase, plays a crucial role in nervous system and potential heart development. Upon binding to its ligand NTF3/neurotrophin-3, TrkC undergoes autophosphorylation, initiating various signaling pathways such as the phosphatidylinositol 3-kinase/AKT and the MAPK pathways. These pathways are instrumental in regulating cell survival and differentiation, highlighting the pivotal role of TrkC in orchestrating essential cellular processes during development.

1.Imobilized human Trkc-His at 2 μg/mL (100 μl/well )can bind biotinylated human NT3, The EC₅₀ of biotinylated human NT3 is ≤ 0.07 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human NT-3 / Neurotrophin-3 Protein at 2 μ g/mL (100 μL/well) can bind Human TrkC Protein. The ED₅₀ for this is ≤4.834 ng/mL.

Human

HEK293

C-10*His

Q16288-2/NP_001007157.1 (C32-D428)

4916  [NCBI]

CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNASINITDISRNITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHLRYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGEAKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGFTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNPPPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLIAKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEED

Approximately 70-100 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% Trehalose, 5% Manitol, 0.01% Tween-80 or 20 mM PB, 150 mM Nacl, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.