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Tryptase beta-2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P71131
COA Handling Instructions

Tryptase beta-2, a neutral protease abundant in mast cells, plays a key role in innate immunity.As the primary proteolytic enzyme released during mast cell activation, Tryptase beta-2 contributes significantly to immune functions.Its involvement underscores its importance in mediating cellular responses linked to innate immunity, highlighting its role in the intricate defense mechanisms of the immune system.Tryptase beta-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tryptase beta-2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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10 μg $120 Get quote
50 μg $340 In-stock
100 μg   Get quote  

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  • Biological Activity

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  • Documentation

Description

Tryptase beta-2, a neutral protease abundant in mast cells, plays a key role in innate immunity.As the primary proteolytic enzyme released during mast cell activation, Tryptase beta-2 contributes significantly to immune functions.Its involvement underscores its importance in mediating cellular responses linked to innate immunity, highlighting its role in the intricate defense mechanisms of the immune system.Tryptase beta-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tryptase beta-2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Tryptase beta-2, a neutral protease primarily found in mast cells, assumes a significant role in innate immunity. It acts as the major proteolytic enzyme released during the activation-degranulation response of mast cells, contributing to their immune functions. The involvement of Tryptase beta-2 underscores its importance in mediating cellular responses associated with innate immunity, emphasizing its role in the complex and dynamic defense mechanisms of the immune system.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P21845 (A22-S276)

Gene ID
Molecular Construction
N-term
TPSB2 (A22-S276)
Accession # P21845
6*His
C-term
Synonyms
Tryptase beta-2; Tryptase-2; Mast cell protease 6; mMCP-6
AA Sequence

APRPANQRVGIVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWVLTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHPISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGMLCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS

Molecular Weight

32-38 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM PB, 50% glycerin, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Tryptase beta-2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Tryptase beta-2 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P71131
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